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The aim of this study was to evaluate the effects of N-acetylcysteine (NAC) on hepatic ischaemia/reperfusion injury (IRI). Adult male Sprague-Dawley rats were divided into three groups: a sham operation group (S group), an IR group and an NAC-treated IR (N group). The hepatic IRI model was established by clamping the hepatic hilus for 45 min and reperfusion for 6h. The N group was treated with NAC (2.5g/kg i.v.), whereas the S and IR groups were administered an equivalent volume of 0.9% saline (0.5ml/kg i.v.). Blood samples and livers were collected at 1, 6 and 24h after reperfusion. Serum levels of alanine transaminase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were determined by enzymatic methods. The activity of inducible nitric oxide synthase (iNOS) in liver tissue was determined by using Griess assay. Histological changes in liver tissue were assessed by haematoxylin-eosin (H-E) staining. 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